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The Good Bacteria

In Agriculture: Soil Fertility


Nitrogen is essential for plants for the synthesis of protein, nucleic acids, and other nitrogen containing compounds. Molecular nitrogen (N2) occupies almost 80% of the atmosphere. For use by plants nitrogen must be fixed (combined) with other elements such as oxygen and hydrogen. The plants take nitrogen in the from of nitrates. In soil, nitrates are formed by the activity of bacteria by two processes: 

(i)        By nitrogen-fixing bacteria : Nitrogen-fixing bacteria are found in the soil either freely e.g., Azotobacter, Clostridium or in root nodules of leguminous plant e.g., Bradyrhizobium, Rhizobium leguminosarum. These bacteria are capable of converting atmospheric free nitrogen into nitrates, the compounds which are useful for the growth of plants. The nitrogen fixing bacteria are the natural fertilizers of the soil. Because of their ability of fixing the atmospheric nitrogen, the leguminous plants are commonly used in crop rotation.


(ii)      By nitrifying bacteria : The decay of plant and animal proteins, affected by saprophytic soil bacteria, returns nitrogen to the soil in the form of ammonia. The process is termed ammonification. The ammonia is not readily utilized by plants. The ammonia so produced, reacts with the soil water to form ammonium hydroxide (NH4OH), which in turn, reacts with various chemical substances found in the soil, to form ammonium salts.

In the process of nitrification, the nitrifying bacteria convert the ammonia or ammonium salts to nitrate, available as the principal nitrogen source for green plants.


Nitrification occurs in two steps, the oxidation of ammonia or ammonium compounds to nitrite by nitrite bacteria e.g., Nitrosomonas and Nitrosococcus, etc., and after that the oxidation of nitrite to nitrate by the nitrate bacteria e.g., Nitrobacter







Thus, the ammonifying and nitrifying bacteria maintain he supply of nitrate to the soil




Disposal of Sewage

Sewage contain domestic wastes, human wastes, leaves of plants, industrial wastes and organic food wastes, etc.During the anaerobic phase of decomposition of sewage wastes, methane (CH4) and CO2 are produced by methanogenic bacteria, such as, Methanococcus and Methanobacterium etc. From these gases, methane is used as a biogas as fuel and CO2 is used by some algae, present in the tank for photosynthesis. The oxygen, produced as a result of photosynthesis, is used for the respiration of living organisms of sewage.
The sludge, that contains harmless organic and inorganic materials, and microorganisms can be dried and used as the fertilizers.

In dairy

 Through their metabolic activities, bacteria may play important roles in the manufacture of curd, butter and certain kinds of cheese from milk.


Streptococcus lactis and Lactobacillus lactis, etc. are found in milk. These bacteria ferment lactose sugar found in milk to form lactic acid, by which milk becomes sour. If the milk is heated at 62.8° C for 30 minutes and is simultaneously cooled, the number of lactic acid bacteria is reduced, but all the spores and cells of these bacteria are not destroyed but pathogenic spores are destroyed. In this way, though the milk becomes sour, but it takes longer time to become sour than ordinary milk. This process is known as pasteurization


The manufacture of almost all milk products, such as curd, butter, cheese, ghee is based on the bacterial activity. Lactic acid bacteria bring together droplets of casein, a protein fund in milk and help in the formation of curd. On churning of curd, butter is derived in the form of fat's rounded droplets. The butter on heating is converted into "ghee".


Following bacteria are responsible for dairy product—

Curd
Streptococcus lactis
Cheese
Lactobacillus lactis
Butter
Streptococcus lactis
Yoghurt
Lactobacillus vulgaricus
Buttermilk
S. lactis and S. cremoris


Industrial value

In Vinegar industry :
The commercial production of vinegar (acetic acid) involves bacterial action. In the first step, the carbohydrates undergo fermentation to be converted into ethyl alcohol (C2H5OH) through the action of yeasts. In the second step, the aerobic bacteria e.g., Acetobacter aceti and Mycoderma aceti, oxidize the alcohol to acetic acid.



In tobacco industry :
Bacillus megatherium and Mycrococcus candidans is used for its fermentative capacity for developing flavour and taste in tobacco leaves.

In tea industry :
By fermentative action of Mycrococcus candidans, the curing of tea leaves is done. By this process special flavour and the taste is developed in the tea leaves.

Tanning of skin to produce leather :
Some bacteria by fermentation decompose fats, which are found in skin of animals, with the result that skin and hair are separated from each other and thus leather becomes ready for use.

Role of Bacteria in Genetic Analysis

Bacteria are very useful organisms for genetic analysis. Under favourable conditions of growth and reproduction, some bacteria (such as E.coli) divide after every 20 minutes, the progeny of each division becomes a new generation. A genetic experiment can be completed with bacteria in hours, while an analogous experiment with a multicellular organism would take months or years to be completed because the generation time of such organisms are moths or even years in duration.

Role of Bacteria in the Production of Antibiotics (medicines)

An antibiotic (the wonder drugs of today) is a chemical, that is produced by a living organism and is capable of inhibiting the growth of pathogenic microorganisms. Some of the antibiotics are produced by bacteria. Man has isolated many of the antibiotics from various bacteria and has utilized them as drugs for the treatment of diseases caused by the pathogenic organisms e.g., Bacillus brevis produces antibiotic thyrothricin and B. subtilis produces antibiotic subtelin. One of the best known antibiotic is the penicillin, obtained from the mold Penicillium notatum, was discovered by the British bacteriologist Alexander Fleming in 1928.

The antibiotics are not laboratory chemicals as are the antiseptics or disinfectants. In some cases antibiotics have been synthesised by chemist in the laboratory, in which case they are known as chemotherapeutic agents.